Nature

Comparison of competitive-nested PCR and real-time PCR in detecting BCR-ABL fusion transcripts in chronic myeloid leukemia patients

All peripheral blood or bone marrow samples in this study were derived from CML patients admitted to the MD Anderson Cancer Center from January 1997 to April 2002. Where indicated, only those patient ...
Frontiers

Raising the bar: genus-specific nested PCR improves detection and lineage identification of avian haemosporidian parasites

Avian haemosporidian parasites are useful model organisms to study the ecology and evolution of parasite-host interactions due to their global distribution and extensive biodiversity. Detection of ...
Scientific Research Publishing

Contribution of Nested PCR for the Diagnosis of the Immunodeficiency Virus and the Detection of Endogenization of the Provirus in Serodiscordant Couples in Kinshasa, Democratic ...

1 Makala General Reference Hospital, Kinshasa, Democratic Republic of the Congo. 2 Department of Molecular Biology, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of the ...
Nature

Establishment of two quantitative nested qPCR assays targeting the human EPO transgene

For ethical and safety reasons it is critical to develop easily implemented assays with high sensitivity and specificity for gene doping surveillance. Two nested quantitative real-time PCR (qPCR) ...
International Livestock Research Institute

A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals

Theileria parva causes East Coast fever, an economically important disease of cattle in sub-Saharan Africa. We describe a nested polymerase chain reaction (nPCR) assay for the detection of T. parva ...
JSTOR Daily

Sensitive and Quantitative Detection of Severe Acute Respiratory Syndrome Coronavirus Infection by Real-Time Nested Polymerase Chain Reaction

A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale ...
Frontiers

Single cell PCR amplification of diatoms using fresh and preserved samples

1 Research and Collections, Canadian Museum of Nature, Ottawa, ON, Canada 2 Biology and Environmental Science, Center for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON, ...
C&EN

The End of the Gray Zone: One-Tube Nested Recombinase Polymerase Amplification with Ultrahigh Signal-to-Noise Ratio for Precisely Detecting and Surveilling Viruses

Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, Sichuan 400044, PR China State Key Laboratory of Cattle ...