Figure 1: Enzymatic cascade of the pyrosequencing reaction in the example of a bisulfite-treated template sequence, including a CpG position that is methylated on approximately 50% of all molecules.

Pyrosequencing can be used to detect DNA and RNA from almost any source, and has applications in bacteria resistance screening, drug development, epigenetics, and many other fields. If no light is ...

Similar to soil and sediment, activated sludge (AS) is a highly complex system of eukaryotes (protozoa and fungi), bacteria, archaea and viruses, in which bacteria are dominant. Despite its importance ...

Multiple fields of microbiology rely on techniques of microbial identification, such as in food microbiology for detecting contaminants, and in microbial ecology to characterize biodiversity. Image ...

The sequencing of DNA molecules started with the development of chemical cleavage method of Maxam-Gilbert during 1970s followed by di-deoxy chain termination method. However, the whole genome ...

Rabies is a fatal zoonosis caused by a nonsegmented negative-strand RNA virus, namely, rabies virus (RABV). Apart from RABV, at least 10 additional species are known as rabies-related lyssaviruses ...

DNA sequencing is one of the most important platforms for the study of biological systems today. Sequence determination is most commonly performed using dideoxy chain termination technology. Recently, ...

Bioelectrochemical systems (BESs) are promising technologies for energy and products recovery coupled with wastewater treatment and have attracted increasing attention (Liu and Logan, 2004; Lovley, ...

Cattle nutrition changes fundamentally from birth to adulthood. Cattle are typical foregut fermenters undergoing considerable changes in anatomy and physiology of the gastrointestinal tract during the ...

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