Figure 1: Enzymatic cascade of the pyrosequencing reaction in the example of a bisulfite-treated template sequence, including a CpG position that is methylated on approximately 50% of all molecules.

DNA methylation is a form of epigenetics in which gene expression is encouraged or inhibited by the addition of a methyl group on cytosines. It is highly implicated in development, cell ...

Pyrosequencing can be used to detect DNA and RNA from almost any source, and has applications in bacteria resistance screening, drug development, epigenetics, and many other fields. If no light is ...

Pyrosequencing as implemented by Roche's 454 is a technology that generates a large number of intermediate length DNA reads through a massively parallel sequencing-by-synthesis approach 1. The GS FLX ...