The enzyme RT of HIV-1 is responsible for copying the viral single-stranded RNA genome to double-stranded DNA (dsDNA) in the cytoplasm of infected cells. This 117-kDa heterodimeric (p66 and p51) ...

In higher eukaryotic genomes, Long Interspersed Nuclear Element 1 (LINE-1) retrotransposons represent a large family of repeated genomic elements. They transpose using a reverse transcriptase (RT), ...

In this study, we demonstrate a strategy using a combination of NMR-based binding and functional assays to screen a library of low-molecular-weight compounds known as fragments to identify new drug ...

The mechanism of action of NNRTIs is illustrated in Figure 1. Reverse transcriptase catalyzes RNA-dependent and DNA-dependent polymerase activities, which are essential for viral DNA synthesis when ...

Single turnover studies on HIV reverse transcriptase suggest that nucleoside analogs bind more tightly to the enzyme than normal substrates, contrary to rational structural predictions. Here we ...

RNA polymerases are sloppy. A retrovirus with an RNA genome, which is copied into DNA by a reverse transcriptase, is prone to mutations because the enzyme lacks the 3’ to 5’ proofreading capability of ...

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One source of endogenous reverse transcriptase (eRT) activity in nucleated cells is the LINE-1/L1 (long interspersed nuclear element-1), a non-LTR retrotransposon that is implicated in the regulation ...

One of the hallmarks of many DNA polymerases is that they proofread as they copy DNA, ensuring high-fidelity duplicates of an organism’s genome. This error-checking ability is missing from enzymes ...

1 National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada 2 National Veterinary Research Institute, Vom, Plateau State, Nigeria Foot-and-Mouth Disease Virus ...