Single-cell omic technologies such as single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) have transformed our ability to dissect cellular heterogeneity within complex tissues.

Advances in next-generation sequencing (NGS) technologies have lowered costs and reduced the complexity of DNA and RNA sequencing, enabling new possibilities when studying gene activity. In ...

The liver is remarkable for its ability to regenerate after injury, yet when this process fails, acute liver failure (ALF) carries devastating outcomes. Traditional research methods, reliant on bulk ...

Initially, cells are dissociated from liver tissues into single-cell suspensions using in vivo enzymatic perfusion or ex vivo digestion methods. The cells of interest are then enriched from the ...

Immune low-response states significantly impact the effectiveness of immunotherapy in cancer treatment. These states ...

A comprehensive review article titled “Bioinformatics perspectives on transcriptomics: A comprehensive review of bulk and single-cell RNA sequencing analyses,” published in Quantitative Biology, ...