Construct a restriction map for a circular molecule of DNA, 40 ... | Filo
Construct a restriction map for a circular molecule of DNA, 40 ... | Filo
Acum 7 luni
askfilo.com
SOLVED: Construct a restriction map of a linear fragment of DNA, using the following data Your map should indicate the relative positions of the restriction sites along with distances from the ends of the molecule to the restriction sites and between restriction sites:DNA uncut DNA DNA cut with EcoRI DNA cut with Hindlll DNA cut with BamHI DNA cut with EcoRI Hindlll DNA cut with EcoRI BamHI DNA cut with Hindlll BamHISizes of Fragments (bp) 900 700, 200 600, 300 500,350, 50 600,200, 100 500, 200,
SOLVED: Construct a restriction map of a linear fragment of DNA, u…
Acum 2 luni
numerade.com
a. Given the following restriction map of a cloned 10 kb piece of DNA, what size fragments would you see after digesting this linear DNA fragment with each of the enzymes or combinations of enzymes listed? (1) EcoRI, (2) BamHI, (3) EcoRI + HindIII, (4) BamHI + Pstl, and (5) EcoRI + BamHI. b. What fragments in the last three double digests would hybridize on a Southern blot with a probe made from the 4 kb Bam HI fragment? | Numerade
a. Given the following restriction map of a cloned 10 kb piece of DN…
Acum 1 lună
numerade.com
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