Top suggestions for modify [The retention time for the product is not correct. I double checked the method, and it is correct. Before I used LCMS-6, the previous operator used formic acid in water for channel A instead of 20 mM of AA. Before I started my run, I switched channel A to 20 mM of AA and rinsed the entire fluic pathway for > 30min. Maybe I need to flush fluidic pathway for longer time. Not sure whether this caused the retention time shift. ] |
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